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Anti-PXN Antibody E228
|A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:5000; IHC: 1: 100-1:250; ICC: 1:250; IP: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - Paxillin (phospho Y31) antibody [E228]; All lanes : Anti-Paxillin (phospho Y31) antibody [E228] at 1/10000 dilution.Lane 1 : untreated NIH 3T3 cell lysate.Lane 2 : PDGF treated NIH 3T3 cell lysate.Predicted band size : 64 kDa.Observed band size : 64 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Paxillin (phospho Y31) antibody [E228]; Immunohistochemical analysis of Paxillin (phospho Y31) expression in paraffin-embedded human colon carcinoma using 1/100 TA300327.
Immunocytochemistry/ Immunofluorescence - Paxillin (phospho Y31) antibody [E228]; Immunofluorescent analysis of Paxillin (phospho Y31) expression in HeLa cell culture, using 1/250 TA300327.
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody [E228]; Immunofluorescence analysis of HeLa cells, staining Paxillin (phospho Y31) with TA300327. Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.