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Anti-PUM1 Antibody EPR3795
Also for PUM1 (NM_001020658)
|A synthetic peptide corresponding to residues in human Pumilio-1 was used as an immunogen. This antibody is predicted to not cross react with Pumilio-2.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; IP: 1:50; FC: 1:100; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens pumilio RNA-binding family member 1 (PUM1), transcript variant 1|
|HSPUM; PUMH; PUMH1; PUML1|
|Pumilio-1 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. It belongs to the PUF family, 84% identical to Pumilio-1. It is required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts (1). Pumilio-1 is highly expressed in testis and ovary and is predominately expressed in stem cells and germ cells. |
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Western blot - Pumilio 1 antibody [EPR3795]; All lanes : Anti-Pumilio 1 antibody [EPR3795] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysates .Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 126 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pumilio 1 antibody [EPR3795]; Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Pumilio 1 antibody [EPR3795]; Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.
Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795]; Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.