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Anti-PTK2 PHOSPHO Antibody EP1832Y
Also for PTK2 (NM_153831)
|A phospho-specific peptide corresponding to residues surrounding Tyrosine 576/577 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y576/577.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:100000; IP: 1:40; ICC: 1:50 - 1:100; FC: 1:20
|Does not react with Rat. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IHC-P.
|Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1|
|FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71|
|Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). |
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Western blot - FAK (phospho Y576 + Y577) antibody [EP1832Y]; All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] at 1/50000 dilution.Lane 1 : NIH-3T3 cell lysates, untreated.Lane 2 : NIH-3T3 cell lysates treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 119 kDa.Observed band size : 125 kDa .