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Anti-PTK2 Antibody EP1831Y
Also for PTK2 (NM_153831)
|A synthetic peptide corresponding to residues in human FAK were used as an immunogen|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 2000; IHC: 1: 100 250; ICC: 1: 100 250; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 1|
|FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71|
|Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). |
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Western blot - FAK antibody [EP1831Y]; Anti-FAK antibody [EP1831Y] at 1/500 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labeled goat anti-rabbit at 1/1000 dilution.Predicted band size : 119 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP1831Y]; Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 ab76496