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Anti-PTK2 Antibody EP695Y
Also for PTK2 (NM_153831)
|A synthetic peptide corresponding to residues in human FAK was used as immunogen.|
|Mouse, Rat, Cow, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||FC: 1:100; ICC/IF: Use at an assay dependent concentration; IHC-P: Use at an assay dependent dilution; ICC: 1:250 - 1:500; WB: 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - FAK antibody [EP695Y]; Anti-FAK antibody [EP695Y] at 1/1000 dilution + Hela cell lysate.Predicted band size : 119 kDa.Observed band size : 119 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using TA300620.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAK antibody [EP695Y]; The image shows FAK antibody in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.
Immunofluorescence - FAK antibody [EP695Y]; Immunofluorescent staining of HeLa cells using TA300620.
Flow Cytometry-FAK antibody [EP695Y](TA300620); Overlay histogram showing HeLa cells stained with TA300620 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.