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Home Antibody All anti-PSMD3 antibodies

Anti-PSMD3 TRUEMAB Antibody clone 2A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504337
  • PSMD3 mouse monoclonal antibody,clone 2A2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400996) , 20ug
100ul 379 In Stock
CF504337
  • Carrier-free (BSA/glycerol-free) PSMD3 mouse monoclonal antibody,clone 2A2
100ug $450 3-4 weeks
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WB(2)
FC(3)
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Also for PSMD3 (NM_002809)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PSMD3(NP_002800) produced in HEK293T cell.
Clone Nameclone 2A2 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.95 mg/ml
Guaranteed Application *WB, FC Suggested Dilutions WB 1:500~2000, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)
Alternative NameP58; RPN3; S3; TSTA2
Database LinkNP_002800
FunctionThe 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq, Jul 2008].
Related Pathway
Cell cycle

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD3 (RC202307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
FC Image
HEK293T cells transfected with either RC202307 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMD3 antibody(TA504337), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PSMD3 antibody(TA504337),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PSMD3 antibody(TA504337),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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