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Anti-PSMB4 TRUEMAB Antibody clone 5B5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PSMB4 (NM_002796)
|Full length human recombinant protein of human PSMB4(NP_002787) produced in HEK293T cell.|
|WB, IF, FC
|| WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens proteasome (prosome, macropain) subunit, beta type, 4 (PSMB4)|
|HN3; HsN3; PROS-26; PROS26|
|The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB4 (RC205723, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB4.
Anti-PSMB4 mouse monoclonal antibody (TA503480) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB4(RC205723).
HEK293T cells transfected with either RC205723 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMB4 antibody(TA503480), and then analyzed by flow cytometry.