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Anti-PSMA6 TRUEMAB Antibody Clone 1F3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PSMA6 (NM_002791)
|Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.|
|Human , Dog , Rat , Monkey , Mouse
||WB 1:500~1000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 6 (PSMA6), transcript variant 1|
|IOTA; p27K; PROS27|
|The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800104), and then analyzed by flow cytometry.