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Anti-PSEN1 Antibody EP2000Y
Also for PSEN1 (NM_000021)
|A synthetic peptide corresponding to residues in human Presenilin 1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:5000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:20; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens presenilin 1 (PSEN1), transcript variant 1|
|AD3; FAD; PS-1; PS1; S182|
Entrez Gene 5663 Human
Entrez Gene 19164 Mouse
Entrez Gene 29192 Rat
|Presenilin (PS) facilitates gamma-secretase cleavage of the beta-amyloid precursor protein and the intramembraneous cleavage of Notch1 (1). Presenilin (PS) is essential for the gamma-cleavage required for the generation of the C terminus of amyloid beta-protein (Abeta). The discovery that a deficiency of presenilin 1 (PS1) decreases the production of amyloid beta-protein (Abeta) identified the presenilins as important mediators of the gamma-secretase cleavage of beta-amyloid precursor protein (APP). It has been shown that two conserved transmembrane (TM) aspartates in PS1 are critical for Abeta production, providing evidence that PS either functions as a required diaspartyl cofactor for gamma-secretase or is itself gamma-secretase. Presenilin 2 (PS2) shares substantial sequence and possibly functional homology with PS1. It has been shown that the two TM aspartates in PS2 are also critical for gamma-secretase activity, providing further evidence that PS2 is functionally homologous to PS1 (2). PS are found primarily within intracellular membranes, including the endoplasmic reticulum (ER) and the trans-Golgi network as well as the plasma membrane. PS are also expressed in most cell types throughout development (3). |
|TransmembraneProteaseDruggable Genome Wnt signaling pathwayNotch signaling pathwayNeurotrophin signaling pathwayAlzheimer's disease|
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Western blot - Presenilin 1 antibody [EP2000Y]; Anti-Presenilin 1 antibody [EP2000Y] at 1/5000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit HRP labelled antibody at 1/2000 dilution.Predicted band size : 53 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Presenilin 1 antibody [EP2000Y]; Immunohistochemical staining of paraffin-embedded human liver, using TA300982, at 1/100-1/250 dilution.
Flow Cytometry-Anti-Presenilin 1 antibody [EP2000Y](TA300982); Overlay histogram showing HepG2 cells stained with TA300982 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.