Home Antibody All anti-PRSS50 antibodies
Anti-PRSS50 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PRSS50 (NM_013270)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
|| WB 1:5000~20000, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens protease, serine, 50 (PRSS50)|
|TSP50 is a possible protease in human testes. It may function in mammalian spermatogenesis. Its linear catalytic structure is similar to many serine proteases, except for a Thr-for-Ser residual catalytic site substitution.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRSS50 (RC205514, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRSS50.
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemistry: TSP50 Antibody - Strong cytoplasmic positivity was seen in primary spermatocytes of the seminiferous tubules of the testes, neurons of the cerebral cortex and many hematopoeitic cells of the bone marrow. Most mucosal surfaces and glands showed moderate or some times weak positivity. Biliary ductal cells and gall bladder mucosal cells showed strong accentuation of the luminal cell membrane. Most other normal tissues showed moderate or weak positivity. More consistent and stronger expression was seen in malignancies. Strong or moderate positivity was seen in prostatic, testicular and colo-rectal cancers. Many pancreatic, prostatic and some testicular cancers showed strong cytoplasmic granularity. Strong or moderate positivity was also seen in malignant carcinoid, renal and urothelial cancers. Most other cancers showed moderate or sometimes weak positivity. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.