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Anti-PRMT5 Antibody EPR5772
Also for PRMT5 (NM_001039619)
|A synthetic peptide corresponding to residues in human PRMT5 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, IF, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens protein arginine methyltransferase 5 (PRMT5), transcript variant 2|
|HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs|
Entrez Gene 10419 Human
Entrez Gene 27374 Mouse
Entrez Gene 364382 Rat
|PRMT5 is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. It specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. PRMT5 plays a role in the assembly of snRNP core particles as well as in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation (1). |
|Stem cell - Pluripotency |
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Western blot - PRMT5 antibody [EPR5772]; All lanes : Anti-PRMT5 antibody [EPR5772] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : NIH3T3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 73 kDa.
Other-Anti-PRMT5 antibody [EPR5772](TA310782); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT5 antibody [EPR5772]; TA310782 at 1/100 dilution staining PRMT5 in Mouse Kidney by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-PRMT5 antibody [EPR5772]; ICC/IF image of TA310782 stainedHepG2 cells. The cells were 100%methanol fixed (5 min) and then incubated in 1%BSA / 10% normalgoat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was , DyLight? 488goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Anti-PRMT5 antibody [EPR5772](TA310782); Overlay histogram showing HeLa cells stained with TA310782 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.