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Anti-PRKCZ PHOSPHO Antibody EP1491Y
Also for PRKCZ (NM_002744)
|A phospho specific peptide corresponding to residues surrounding Threonine 410 and 403 of human PKC zeta/lambda. This antibody detects PKC zeta/lambda phosphorylated at T410/403.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1: 1000; IHC: 1: 100~250; ICC: 1: 100~250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1|
|The atypical protein kinase C isoform, PKCzeta, has been implicated in the control of extracellular signal-regulated kinase (ERK) and nuclear factor (NF)-kappa B pathways. It has been shown that the loss of PKCzeta selectively impairs signaling through the B-cell receptor, resulting in inhibition of cell proliferation and survival, as well as defects in the activation of ERK and the transcription of NF-kappa B-dependent genes (1). The lack of PKCzeta in embryonic fibroblasts (EFs) severely impairs kappaB-dependent transcriptional activity as well as cytokine-induced phosphorylation of p65. Also, a cytokine-inducible interaction of PKCzeta with p65 was detected which requires the previous degradation of IkappaB (2). It has been found that persistent PKCzeta activity maintains potentiated responses, not only of the strongly tetanized pathway, but also of the weakly tetanized pathway. In contrast, an independent, nontetanized pathway was unaffected by the inhibitor, indicating that the function of PKCzeta was specific to the tagged synapses (3). |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - PKC zeta (phospho T403 + T410) antibody [EP1491Y]; All lanes : Anti-PKC zeta (phospho T410 + T412) antibody [EP1491Y] at 1/2000 dilution.Lane 1 : 293 cell lysates, untreated.Lane 2 : 293 cell lysates, treated with AP.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit-HRP at 1/1000 dilution.Predicted band size : 68 kDa.Observed band size : 68 kDa.Additional bands at : 165 kDa,170 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC zeta (phospho T403 + T410) antibody [EP1491Y]; ab76129 at 1/100 dilution staining PKC zeta in human brain.
Immunocytochemistry/ Immunofluorescence - PKC zeta (phospho T403 + T410) antibody [EP1491Y]; ab76129 at 1/100 dilution staining PKC zeta in HeLa cells by Immunofluorescence.