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Anti-PRKCE PHOSPHO Antibody
Also for PRKCE (NM_005400)
|The antiserum was produced against synthesized phosphopeptide derived from human PKC e around the phosphorylation site of serine 729 (G-F-SP-Y-F).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|PKC . (Phospho-Ser729) antibody detects endogenous levels of PKC . only when phosphorylated at serine 729.
|Homo sapiens protein kinase C, epsilon (PRKCE)|
Wnt Signaling Pathway
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Western blot analysis of extracts from HeLa cells, Jurkat cells and NIH/3T3 cells all treated with PMA (125ng/ml, 30mins), using PKC e (Phospho-Ser729) antibody.The lane on the right is treated with the synthesized peptide.