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Home Antibody All anti-PRKCD antibodies

Anti-PRKCD PHOSPHO Antibody EP2609Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301278
  • Rabbit Monoclonal Antibody against PRKCD (Clone EP2609Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Tyrosine 311 of human PKC-delta was used as an immunogen. The antibody only detects PKC-delta phosphorylated on Tyrosine 311.
Clone NameEP2609Y IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2500 - 1:5000; IP: 1:40; IHC-P: 1:100
BufferDoes not react with Rat. Is unsuitable for Flow Cyt or ICC/IF.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or ICC/IF.

Reference Data

Target NameHomo sapiens protein kinase C, delta (PRKCD), transcript variant 1
Alternative NameCVID9; MAY1; nPKC-delta; PKCD
Database LinkNP_006245
Entrez Gene 5580 Human
Entrez Gene 18753 Mouse
FunctionPKC-delta has a biological activity which is closely related to Pkc1. PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). PKC-delta is phosphorylated on tyrosine residues in response to thrombin and collagen, and it has recently been shown that Tyrosine 311 is phosphorylated in response to protease-activated receptor 1 and protease-activated receptor 4 activation (3).
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayVascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisNeurotrophin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PKC delta (phospho Y311) antibody [EP2609Y]; All lanes : Anti-PKC delta (phospho Y311) antibody [EP2609Y] at 1/5000 dilution.Lane 1 : U937 cell lysate.Lane 2 : U937 cell lysate treated with TPA.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit-HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 78 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC delta (phospho Y311) antibody [EP2609Y]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with TA301278 at 1/100 dilution.

 

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