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Anti-PRKCD PHOSPHO Antibody EP2609Y
Also for PRKCD (NM_006254)
|A phospho-specific peptide corresponding to residues surrounding Tyrosine 311 of human PKC-delta was used as an immunogen. The antibody only detects PKC-delta phosphorylated on Tyrosine 311.|
||Tissue culture supernatant
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2500 - 1:5000; IP: 1:40; IHC-P: 1:100
|Does not react with Rat. Is unsuitable for Flow Cyt or ICC/IF.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or ICC/IF.
|Homo sapiens protein kinase C, delta (PRKCD), transcript variant 1|
|CVID9; MAY1; nPKC-delta; PKCD|
Entrez Gene 5580 Human
Entrez Gene 18753 Mouse
|PKC-delta has a biological activity which is closely related to Pkc1. PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). PKC-delta is phosphorylated on tyrosine residues in response to thrombin and collagen, and it has recently been shown that Tyrosine 311 is phosphorylated in response to protease-activated receptor 1 and protease-activated receptor 4 activation (3). |
|Protein KinaseDruggable Genome Chemokine signaling pathwayVascular smooth muscle contractionTight junctionFc epsilon RI signaling pathwayFc gamma R-mediated phagocytosisNeurotrophin signaling pathwayMore Pathways >> |
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Western blot - PKC delta (phospho Y311) antibody [EP2609Y]; All lanes : Anti-PKC delta (phospho Y311) antibody [EP2609Y] at 1/5000 dilution.Lane 1 : U937 cell lysate.Lane 2 : U937 cell lysate treated with TPA.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit-HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 78 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC delta (phospho Y311) antibody [EP2609Y]; Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with TA301278 at 1/100 dilution.