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Anti-PRKCA PHOSPHO Antibody
Also for PRKCA (NM_002737)
|The antiserum was produced against synthesized phosphopeptide derived from human PKC-pan around the phosphorylation site of threonine 497 (T-S-TP-F-C).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|PKC-pan (Phospho-Thr497) antibody detects endogenous levels of PKC-pan only when phosphorylated at threonine 497.
* Availability is in business days
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Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKC-pan (Phospho-Thr497) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC-pan (Phospho-Thr497) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) antibody.The picture on the right is treated with the synthesized peptide.