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Home Antibody All anti-PRKCA antibodies

Anti-PRKCA Antibody Y124

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303658
  • Rabbit monoclonal antibody against PKCa (C-term)(Y124)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400966) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human PKC a was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Clone NameY124 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:250 - 1:500; FC: 1:100; IP: 1:100; IHC-Fr: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens protein kinase C, alpha (PRKCA)
Alternative NameAAG6; PKC-alpha; PKCA; PRKACA
Database LinkNP_002728
FunctionProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
MAPK signaling pathway
Melanogenesis
Wnt Signaling Pathway

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WB Image
Western blot - PKC alpha antibody [Y124]; Anti-PKC alpha antibody [Y124] at 1/10000 dilution + 293 cell lysate.Predicted band size : 77 kDa.Observed band size : 75 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha antibody [Y124]; Ab32376, at a 1/250 dilution, staining PKC alpha in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [Y124]; Immunohistochemical analysis of mouse small intestine tissue, staining PKC alpha with TA303658.
IF Image
Immunocytochemistry/ Immunofluorescence - PKC alpha antibody [Y124]; Ab32376, at a 1/250 dilution, staining PKC alpha in HeLa cells by Immunofluorescence.
IF Image
Immunocytochemistry - PKC alpha antibody [Y124]; Overlay histogram showing HeLa cells stained with TA303658 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
FC Image
Flow Cytometry-PKC alpha antibody [Y124](TA303658); Overlay histogram showing HeLa cells stained with TA303658 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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