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Anti-PRDX3 Antibody EPR8114
Also for PRDX3 (NM_006793)
|A synthetic peptide corresponding to residues in human Peroxiredoxin 3 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||IHC-P: 1:50 - 1:100; WB: 1:50000 - 1:200000; ICC/IF: 1:50 - 1:100
|pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens peroxiredoxin 3 (PRDX3), transcript variant 1|
|AOP-1; AOP1; HBC189; MER5; PRO1748; prx-III; SP-22|
Entrez Gene 10935 Human
|Peroxiredoxin 3 contains an antioxidant function and is localized in the mitochondrion.It shows significant sequence similarity to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of Peroxiredoxin 3 in E. coli deficient in the C22-subunit protein rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse proteins are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these Peroxiredoxin 3 proteins consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions (1).|
|Transcription Factors |
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Western blot - Anti-Peroxiredoxin 3 antibody [EPR8114]; All lanes : Anti-Peroxiredoxin 3 antibody [EPR8114] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 26 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3 antibody [EPR8114]; TA311040, at 1/50 dilution, staining Peroxiredoxin 3 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3 antibody [EPR8114]; TA311040, at 1/50 dilution, staining Peroxiredoxin 3 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3 antibody [EPR8114]; TA311040, at 1/50 dilution, staining Peroxiredoxin 3 in MCF-7 cells by Immunofluorescence.