OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PRDX2 antibodies

Anti-PRDX2 Antibody EPR5154

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307849
  • Rabbit monoclonal antibody against Peroxiredoxin 2(clone EPR5154)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC405644) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(2)
IF(2)
FC(1)
spacer
Also for PRDX2 (NM_181738)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Peroxiredoxin 2 was used as an immunogen.
Clone NameEPR5154 IsotypeIgG
Species ReactivityMouse, Rat, Human, Pig ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:1000; WB: 1:1000 - 1:10000; IHC-P: 1:1000 - 1:2000; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens peroxiredoxin 2 (PRDX2), nuclear gene encoding mitochondrial protein, transcript variant 3
Alternative NameNKEFB; PRP; PRX2; PRXII; PTX1; TDPX1; TPX1; TSA
Database LinkNP_859428
FunctionPeroxiredoxin 2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. It may play an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Peroxiredoxin 2 antibody [EPR5154]; All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : LnCaP cell lysate.Lane 4 : SH-SYSY cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 22 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 2 antibody [EPR5154]; Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human prostatic hyperplasia tissue using TA307849 at 1/1000 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 2 antibody [EPR5154]; Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human stomach tissue using TA307849 at 1/1000 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 2 antibody [EPR5154]; Immunofluorescent staining of Peroxiredoxin 2 in HeLa cells using TA307849 at 1/250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2 antibody [EPR5154]; TA307849 (1/500) staining Peroxiredoxin 2 in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red).
FC Image
Flow Cytometry - Anti-Peroxiredoxin 2 antibody [EPR5154]; Overlay histogram showing HeLa cells stained with TA307849 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title