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Anti-PRDX2 Antibody EPR5154
Also for PRDX2 (NM_181738)
|A synthetic peptide corresponding to residues in human Peroxiredoxin 2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:1000; WB: 1:1000 - 1:10000; IHC-P: 1:1000 - 1:2000; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens peroxiredoxin 2 (PRDX2), nuclear gene encoding mitochondrial protein, transcript variant 3|
|NKEFB; PRP; PRX2; PRXII; PTX1; TDPX1; TPX1; TSA|
|Peroxiredoxin 2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. It may play an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression.|
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Western blot - Peroxiredoxin 2 antibody [EPR5154]; All lanes : Anti-Peroxiredoxin 2 antibody [EPR5154] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : LnCaP cell lysate.Lane 4 : SH-SYSY cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 22 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 2 antibody [EPR5154]; Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human prostatic hyperplasia tissue using ab109367 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 2 antibody [EPR5154]; Immunohistochemical analysis of Peroxiredoxin 2 in paraffin-embedded Human stomach tissue using ab109367 at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 2 antibody [EPR5154]; Immunofluorescent staining of Peroxiredoxin 2 in HeLa cells using ab109367 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2 antibody [EPR5154]; ab109367 (1/500) staining Peroxiredoxin 2 in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Flow Cytometry - Anti-Peroxiredoxin 2 antibody [EPR5154]; Overlay histogram showing HeLa cells stained with ab109367 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.