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Anti-PPT1 TRUEMAB Antibody Clone OTI1A10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA800490 PPT1 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10) 100ul 379
In Stock
LC400121 PPT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800490 Carrier-free (BSA/glycerol-free) PPT1 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.
Clone NameClone OTI1A10 IsotypeIgG2b
Species ReactivityHuman , Dog , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 31.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1
Alternative NameCLN1; INCL; PPT
Database LinkNP_000301
Entrez Gene 5538 Human
Entrez Gene 693377 Monkey
Entrez Gene 475316 Dog
FunctionThe protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008].
Related PathwayDruggable Genome Fatty acid elongation in mitochondriaMetabolic pathwaysLysosome

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* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1 (RC203278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPT1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800490)


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