Anti-PPP1R15A Antibody, clone 3D12

Also for PPP1R15A (NM_014330)

Gene Name	Homo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Synonyms:	GADD34
Immunogen	Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 3D12	Isotype	IgG2b
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PPP1R15A mouse monoclonal antibody. (TA504310)

IF Image

Anti-PPP1R15A mouse monoclonal antibody (TA504310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A(RC200581).

FC Image

HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504310), and then analyzed by flow cytometry.

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Anti-PPP1R15A TrueMAB Antibody clone 3D12

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