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Home Antibody All anti-PPP1R15A antibodies

Anti-PPP1R15A TRUEMAB Antibody clone 2B11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504309
  • PPP1R15A (GADD34) mouse monoclonal antibody, clone 2B11
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC415358) , 20ug
100ul 379 In Stock
CF504309
  • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 2B11
100ug $450 3-4 Days
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WB(2)
IF(1)
FC(3)
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Also for PPP1R15A (NM_014330)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Clone Nameclone 2B11 IsotypeIgG1
Species ReactivityHuman , Mouse Concentration0.69mg/ml
Guaranteed Application *WB, IF, FC Suggested Dilutions WB 1:500~2000, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein phosphatase 1, regulatory subunit 15A (PPP1R15A)
Alternative NameGADD34
Database LinkNP_055145
FunctionThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IF Image
Anti-PPP1R15A mouse monoclonal antibody (TA504309) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A(RC200581).
FC Image
HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504309), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PPP1R15A antibody(TA504309),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504309),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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