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Anti-PPP1CB Antibody EP1804Y
Also for PPP1CB (NM_002709)
|A synthetic peptide corresponding to residues on human PP1 beta was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:100000 - 1:200000; IP: 1:100; FC: 1:25; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB), transcript variant 1|
|PP-1B; PP1B; PP1beta; PPP1CD|
|Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3). |
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Western blot - Protein Phosphatase 1 beta antibody [EP1804Y]; Anti-Protein Phosphatase 1 beta antibody [EP1804Y] at 1/200000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Protein Phosphatase 1 beta antibody [EP1804Y]; Paraffin embedded human muscle labelled with ab53315 at 1/100 - 1/250 dilution
Flow Cytometry-Anti-Protein Phosphatase 1 beta antibody [EP1804Y](ab53315); Overlay histogram showing HeLa cells stained with ab53315 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.