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Home Antibody All anti-PPP1CA antibodies

Anti-PPP1CA Antibody EP1511Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300850 Rabbit Monoclonal Antibody against PPP1CA (Clone EP1511Y) 100ul $325 In Stock
LC400954 PPP1CA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for PPP1CA (NM_002708)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human PP1 alpha was used as an immunogen.
Clone NameEP1511Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:200000; IP: 1:100; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution; FC: 1:100
Predicted MW Explanation 37 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 1
Alternative NamePP-1A; PP1A; PP1alpha; PPP1A
Database LinkNP_002699
Entrez Gene 5499 Human
Entrez Gene 19045 Mouse
Entrez Gene 24668 Rat
FunctionProtein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).
Related PathwayPhosphataseDruggable Genome Oocyte meiosisVascular smooth muscle contractionFocal adhesionLong-term potentiationRegulation of actin cytoskeletonInsulin signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-PPP1CA + 1CB [EP1511Y] antibody; Anti-PPP1CA + 1CB [EP1511Y] antibody at 1/200000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.

IHC Image
Anti-PPP1CA + 1CB [EP1511Y] antibody; Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.

IF Image
ICC/IF image of TA300850 stained DU145 cells. The cells were incubated with the antibody TA300850 at 1/200 dilution overnight at 4.. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

FC Image
Flow Cytometry - Anti-PPP1CA + 1CBantibody; Overlay histogram showing HeLa cells stained with TA300850 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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