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Anti-PPP1CA Antibody EP1511Y
Also for PPP1CA (NM_002708)
|A synthetic peptide corresponding to residues on human PP1 alpha was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:200000; IP: 1:100; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 1|
|PP-1A; PP1A; PP1alpha; PPP1A|
|Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3). |
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Western blot - Anti-PPP1CA + 1CB [EP1511Y] antibody; Anti-PPP1CA + 1CB [EP1511Y] antibody at 1/200000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
Anti-PPP1CA + 1CB [EP1511Y] antibody; Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + 1CB [EP1511Y] antibody; ICC/IF image of ab52619 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52619 at 1/200 dilution overnight at +4°C. The secondary antibody (green)was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-PPP1CA + 1CB [EP1511Y] antibody; Overlay histogram showing HeLa cells stained with ab52619 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.