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Home Antibody All anti-POLR2B antibodies

Anti-POLR2B Antibody

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SKU Description Amount Price Availability*  
TA308532 Rabbit Polyclonal antibody to POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140kDa) 100ul $325 3-7 Days
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WB(2)
IHC(1)
IF(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 172 of RNA polymerase IIB (Uniprot ID#P30876)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Rat, Rhesus Monkey, Bovine) Concentration0.65mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 134kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rat (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), transcript variant 1
Alternative NamehRPB140; POL2RB; RPB2
Database LinkNP_000929
Entrez Gene 5431 Human
Entrez Gene 289561 Rat
Entrez Gene 694664 Monkey
FunctionThis gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]
Related PathwayTranscription Factors Purine metabolismPyrimidine metabolismMetabolic pathwaysRNA polymeraseHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (30 ug of whole cell lysate). A: BCL-1. 5% SDS PAGE. TA308532 diluted at 1:1000.
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Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. TA308532 diluted at 1:1000.
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Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B(TA308532) antibody at 1:500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B(TA308532) antibody at 1:200 dilution.
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