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Anti-POLR2A PHOSPHO Antibody EP1510Y
Also for POLR2A (NM_000937)
|A phospho synthetic peptide corresponding to residues surrounding serine 1801 of human Pol II was used as an immunogen. This antibody detects Pol II phosphorylated at serine 1801.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:40; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens polymerase (RNA) II (DNA directed) polypeptide A, 220kDa (POLR2A)|
|hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2|
|DNA-dependent RNA polymerase II (Pol II), a complex multisubunit enzyme, is responsible for the transcription of protein-coding genes. This complex interacts with the promoter regions of genes as well as with a variety of elements and transcription factors to determine essentially all of the parameters that govern transcription. A fraction of the large subunit of Pol II is ubiquitinated after exposing cells to UV radiation or cisplatin suggesting that ubiquitination of Pol II plays a role in the recognition and/or repair of damage to actively transcribed genes (1). Evidence demonstrates that pol II transcription termination involves two distinct and temporally separate events. The first event, termed pretermination cleavage (PTC), is mediated by sequence tracts located downstream of the poly(A) site which appear to promote heterogeneous cleavage of the nascent transcript. The second event, in which pol II disengages from the DNA template, requires that polymerase has transcribed both a PTC sequence tract and a functional poly(A) site (2). |
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Western blot - RNA polymerase II (phospho S1801) antibody [EP1510Y]; All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] at 1/200000 dilution.Lane 1 : Human brain lysates, untreated.Lane 2 : Human brain lysates treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 217 kDa.Observed band size : 270 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA polymerase II (phospho S1801) antibody [EP1510Y]; ab76292, at a 1/100 dilution, staining RNA Polymerase II (phospho S1801) in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - RNA polymerase II (phospho S1801) antibody [EP1510Y]; ab76292, at a 1/250 dilution, staining RNA Polymerase II (phospho S1801) in HeLa cells by Immunofluorescence.