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Anti-PML PHOSPHO Antibody EPR6151
|A phospho specific peptide corresponding to residues surrounding Serine 518 of human PML was used as an immunogen. This antibody only detects PML phosphorylated at Serine 518.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens promyelocytic leukemia (PML), transcript variant 11|
|MYL; PP8675; RNF71; TRIM19|
|PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. PML is often involved with retinoic acid receptor alpha associated with acute promyelocytic leukemia (APL) (1).|
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Western blot - Anti-PML Protein (phospho S518) antibody [EPR6151]; All lanes : Anti-PML Protein (phospho S518) antibody [EPR6151] at 1/1000 dilution.Lane 1 : HeLa lysate (untreated).Lane 2 : HeLa lysate treated with Alkaline Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 110 kDa.Observed band size : 110 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151]; TA310766, at a 1/100 dilution, staining Human PML Protein in astrocytoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151]; TA310766, at a 1/50 dilution, staining Human PML Protein in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.