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Anti-PLOD2 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PLOD2 (NM_182943)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|WB, ELISA, IHC
|| WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 1|
|The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLOD2 (RC207610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLOD2.
Western Blot: PLOD2 Antibody - Western Blot was performed using affinity purified SEQer PLOD2 antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLOD2 Rabbit Polyclonal antibody. (TA590314)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PLOD2 Rabbit Polyclonal antibody. (TA590314)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PLOD2 Rabbit Polyclonal antibody. (TA590314)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PLOD2 Rabbit Polyclonal antibody. (TA590314)