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Anti-PLK1 TRUEMAB Antibody Clone OTI3F12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500383 anti-PLK1 mouse monoclonal antibody, clone OTI3F12 (formerly 3F12) 100ul 325
In Stock
LC417592 PLK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500383 Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody, clone OTI3F12 (formerly 3F12) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human PLK1 expression vector
Clone NameClone OTI3F12 IsotypeIgG1
Species ReactivityHuman Concentration1.01 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 68.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
Entrez Gene 5347 Human
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500383)

IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image
HEK293T cells transfected with either RC201795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLK1 antibody(TA500383), and then analyzed by flow cytometry.


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