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Anti-PLCG2 PHOSPHO Antibody EP1405Y
Also for PLCG2 (NM_002661)
|A phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLC?II was used as an immunogen. This antibody detects PLC?II phosphorylated on tyrosine 1197.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000 - 1:20000; IP: 1:40; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IHC-P.
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Western blot - PLCG 2 (phospho Y1197) antibody [EP1405Y]; All lanes : Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] at 1/20000 dilution.Lane 1 : Raji cell lysates un-treated.Lane 2 : Raji cell lysates treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 148 kDa.Observed band size : 150 kDa .