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Home Antibody All anti-PKMYT1 antibodies

Anti-PKMYT1 TRUEMAB Antibody Clone OTI2B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500839 PKMYT1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4) 100ul $325 In Stock
LC401358 PKMYT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500839 Carrier-free (BSA/glycerol-free) PKMYT1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(2)
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Also for PKMYT1 (NM_004203)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell.
Clone NameClone OTI2B4 IsotypeIgG2a
Species ReactivityHuman Concentration0.88 mg/ml
Guaranteed Application *WB, IF, FC Suggested Dilutions IHC 1:100~200, WB: 1:200
Predicted MW Explanation 54.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1
Alternative NameMYT1; PPP1R126
Database LinkNP_004194
Entrez Gene 9088 Human
FunctionThe protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKMYT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Anti-PKMYT1 mouse monoclonal antibody (TA500839) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1(RC222657).
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Flow cytometric Analysis of Hela cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PKMYT1 antibody(TA500839),(Red), compared to a nonspecific negative control antibody,(Blue).
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