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Anti-PIAS1 Antibody EPR2581Y
Also for PIAS1 (NM_016166)
|A synthetic peptide corresponding to residues on the C-terminus of human PIAS1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:10 - 1:100; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein inhibitor of activated STAT, 1 (PIAS1)|
|DDXBP1; GBP; GU/RH-II; ZMIZ3|
|PIAS1 (protein inhibitor of activated STAT) proteins blocks the DNA binding activity of Stat1 and inhibits Stat1-mediated gene activation in response to cytokine stimulation (1). PIAS1 also plays a crucial role as a transcriptional coregulator in the p53 pathway and the steroid hormone signaling pathway and functions as an E3 type small ubiquitin-like modifier (SUMO) ligas, stabilizing the interaction between UBEZ1 and the substrate, and as a SUMO-tethering factor. It is expressed in numerous tissues with highest level in testes. |
Jak-STAT signaling pathway
TGF Beta Signaling Pathway
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Western blot - PIAS1 antibody [EPR2581Y] - Carboxyterminal end; All lanes : Anti-PIAS3 + PIAS1 + PIAS2 [EPR2581Y] antibody at 1/50000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : 293 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : SW40 cell lysate.Lane 5 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 78 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS1 antibody [EPR2581Y] - Carboxyterminal end; ab77231, at a 1/100 dilution, staining PIAS1 in paraffin embedded human tonsil tissue by Immunohistochemistry.