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Anti-PGR PHOSPHO Antibody EP1516Y
|A phospho specific peptide corresponding to residues surrounding serine 190 of human PR was used as an immunogen. This antibody detects PR phophorylated at serine 190.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; IHC-P: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens progesterone receptor (PGR), transcript variant 2|
|This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform B has not been clearly determined. [provided by RefSeq]. |
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Western blot - Progesterone Receptor (phospho S190) antibody [EP1516Y]; All lanes : Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] at 1/10000 dilution.Lane 1 : T47D cell lysates, untreated.Lane 2 : T47D cell lysates, treated with progesterone.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 99 kDa.Observed band size : 99,118 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Progesterone Receptor (phospho S190) antibody [EP1516Y]; ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.