OriGene Logo
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PFN1 antibodies

Anti-PFN1 TRUEMAB Antibody Clone OTI1D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501211 anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI1D5 (formerly 1D5) 100ul
30ul
$325 In Stock
LC417584 PFN1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501211 Carrier-free (BSA/glycerol-free) PFN1 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5) 100ug $450 4 Weeks
Add to Shopping Cart
spacer
WB(4)
IHC(2)
IF(1)
FC(3)
Write Review & Get Rewarded
X

Write a review and get rewarded!

Review this antibody and earn an OriGene coupon or Amazon giftcard

Follow these easy steps to reward yourself:

  1. Sign in to your account, or register for a new account;
  2. Write a review;
  3. Receive a coupon or giftcard once your review is accepted.

Click the button to start writing a review


OriGene Data

ImmunogenFull length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Clone NameClone OTI1D5 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 14.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens profilin 1 (PFN1)
Alternative NameALS18
Database LinkNP_005013
Entrez Gene 5216 Human
Entrez Gene 64303 Rat
Entrez Gene 710753 Monkey
Entrez Gene 607397 Dog
FunctionThe protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.
Related PathwayStem cell - PluripotencyDruggable Genome Regulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (RC202338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.
spacer

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.
spacer

WB Image
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)
spacer

WB Image
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501211, Dilution 1:50)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501211, Dilution 1:50)
spacer

IF Image
Anti-PFN1 mouse monoclonal antibody (TA501211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFN1(RC202338 ).
spacer

FC Image
HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody(TA501211), and then analyzed by flow cytometry.
spacer

FC Image
Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody(TA501211),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
spacer

FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody(TA501211),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge