Homo sapiens phosphatidylethanolamine binding protein 1 (PEBP1)
Synonyms:
HCNP; HCNPpp; PBP; PEBP; PEBP-1; RKIP
Immunogen
A phospho-specific peptide corresponding to residues surrounding serine 153 of human RKIP was used as an immunogen. This antibody detects RKIP phosphorylated on serine 153.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine-binding protein (PEBP) family. It is an inhibitor of the Raf/MEK/MAP kinase signaling cascade and is a suppressor of cancer metastasis (1). RKIP is involved in neural development, cardiac function and spermatogenesis and appears to have serine protease activity. The inhibition Raf-1-mediated phosphorylation of MEK results from RKIP binding to Raf-1. Dysregulated RKIP expression has the potential to contribute to pathophysiological processes including Alzheimer's disease and diabetic nephropathy (2). RKIP represents a novel effector of signal transduction pathways leading to apoptosis and a prognostic marker of the pathogenesis of human cancer cells and tumors (3). RKIP is a physiological inhibitor of GRK-2. After stimulation of GPCR, RKIP dissociates from its known target, Raf-1 (refs 6-8), to associate with GRK-2 and block its activity. This switch is triggered by protein kinase C (PKC)-dependent phosphorylation of the RKIP on serine 153 (4).
Related Pathway
EGFR1 Signaling Pathway
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Western blot analysis on HeLa cell lysates using anti-Phospho-RKIP (pS153), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
