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Anti-PEBP1 PHOSPHO Antibody EP2845Y
Also for PEBP1 (NM_002567)
|A phospho-specific peptide corresponding to residues surrounding serine 153 of human RKIP was used as an immunogen. This antibody detects RKIP phosphorylated on serine 153.|
|Rat, Human (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens phosphatidylethanolamine binding protein 1 (PEBP1)|
|HCNP; HCNPpp; HEL-210; HEL-S-34; PBP; PEBP; PEBP-1; RKIP|
Entrez Gene 5037 Human
Entrez Gene 29542 Rat
|Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine-binding protein (PEBP) family. It is an inhibitor of the Raf/MEK/MAP kinase signaling cascade and is a suppressor of cancer metastasis (1). RKIP is involved in neural development, cardiac function and spermatogenesis and appears to have serine protease activity. The inhibition Raf-1-mediated phosphorylation of MEK results from RKIP binding to Raf-1. Dysregulated RKIP expression has the potential to contribute to pathophysiological processes including Alzheimer's disease and diabetic nephropathy (2). RKIP represents a novel effector of signal transduction pathways leading to apoptosis and a prognostic marker of the pathogenesis of human cancer cells and tumors (3). RKIP is a physiological inhibitor of GRK-2. After stimulation of GPCR, RKIP dissociates from its known target, Raf-1 (refs 6-8), to associate with GRK-2 and block its activity. This switch is triggered by protein kinase C (PKC)-dependent phosphorylation of the RKIP on serine 153 (4). |
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Western blot - PBP (phospho S153) antibody [EP2845Y]; All lanes : Anti-PBP (phospho S153) antibody [EP2845Y] at 1/2000 dilution.Lane 1 : HeLa cell lysates un-treated.Lane 2 : HeLa cell lysates treated with calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 21 kDa.Observed band size : 21 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBP (phospho S153) antibody [EP2845Y]; TA301282, at 1/100 dilution, staining PBP in human liver by immunohistochemistry using paraffin-embedded tissue.