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Anti-PDPK1 PHOSPHO Antibody EPR336(2)
Also for PDPK1 (NM_031268)
|A phospho specific peptide corresponding to residues surrounding Serine 241 of human PDK1 was used as an immunogen. This antibody only detects PDK1 phosphorylated at Serine 241.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), transcript variant 2|
|PDK1; PDPK2; PRO0461|
|3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).|
Signaling by GPCR
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Western blot - PDPK1 (phospho S241) antibody [EPR336(2)]; All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate .Lane 2 : 293T cell lysate treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 (phospho S241) antibody [EPR336(2)]; ab109460, at a 1/100 dilution, staining PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.