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Anti-PDK2 Antibody EPR1948Y
Also for PDK2 (NM_002611)
|A synthetic peptide corresponding to residues near the N-terminus of human PDK2 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:60; IP: 1:30
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens pyruvate dehydrogenase kinase, isozyme 2 (PDK2), transcript variant 1|
|A member of Pyruvate dehydrogenase kinase (PDK1, 2, 3, and 4), PDK2 is a serine kinase which is involved in the regulation of mammalian glucose metabolism. During aerobic energy metabolism, mitochondrial pyruvate dehydrogeanse complex (PDC) catalyzes decarboxylation of pyruvate to form acetyl-CoA (1). PDK2 inhibits PDC thru phosphorylation of the E1 alpha subunit of PDC and inactivates the complex (2). Highly expressed in heart and skeletal muscles, PDK2 is also responsible for the phosphorylation of Akt at Ser473, which leads to phosphorylation by PDK1 on Thr308 on Akt and full Akt activation (3). |
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Western blot - PDK2 antibody [EPR1948Y]; All lanes : Anti-PDK2 [EPR1948Y] antibody at 1/2000 dilution.Lane 1 : human heart tissue lysate.Lane 2 : mouse heart tissue lysate.Lane 3 : rat tissue lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 46 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK2 antibody [EPR1948Y]; TA301140 at 1/100-1/250 dilution staining PDK2 in human muscle tissue sections.