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Home Antibody All anti-PDHA1 antibodies

Anti-PDHA1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308687
  • Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400110) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for PDHA1 (NM_000284)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration1mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB:1:1000-1:10000; IF:1:100-1:500;
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.

Reference Data

Target NameHomo sapiens pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), transcript variant 1
Alternative NamePDHA; PDHCE1A; PHE1A
Database LinkNP_000275
FunctionThe pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]
Related Pathway

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WB Image
Sample(30 ug of whole cell lysate) A:HepG2 B:MOLT4 (anti-PDHA1 antibody diluted at 1:1000)
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pyruvate Dehydrogenase E1 alpha(anti-PDHA1 antibody) antibody at 1:200 dilution.

 

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