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Home Antibody All anti-PDCD4 antibodies

Anti-PDCD4 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319400
  • Rabbit polyclonal anti-Pdcd4 antibody
100ug 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, Xenopus Concentration1.06 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:10,000 - 1:120,000, WB: 1:1,000 - 1:10,000, IHC: 1:100 – 1:300
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Reference Data

Target NameHomo sapiens programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2
Alternative NameH731
Database LinkNP_663314
Entrez Gene 27250 Human
Entrez Gene 18569 Mouse
Entrez Gene 64031 Rat
Function
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
IHC Image
Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.

 

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