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Anti-PCYT1A Antibody EPR3941
Also for PCYT1A (NM_005017)
|A synthetic peptide corresponding to residues in human CCT-alpha was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
||FC: 1:500 - 1,000, ICC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens phosphate cytidylyltransferase 1, choline, alpha (PCYT1A)|
|CCTA; CT; CTA; CTPCT; PCYT1; SMDCRD|
|CTP:phosphocholine cytidylyltransferase (CCT) is the key regulator enzyme in the synthesis of phosphatidylcholine (PC), the most abundant phospholipid in eukaryotic cell membranes. CCT catalyzes the transfer of a cytidylyl group from CTP to phosphocholine to form CDP-choline (1). A steady, albeit low, production of PC is necessary to maintain suitable PC content for lipid homeostasis. However, elevated activity of CCT is required during cell division when increased PC production is needed for membrane biogenesis (2). To date, three CCT isoforms have been identified: CCT-alpha, CCT-beta 1, and CCT-beta 2 (3). CCT-alpha is localized to the nucleus and nuclear envelope in many cell types. An exception is lung-derived cell, where CCT-alpha is localized primarily in cytoplasmic and ER compartments. CCT-alpha is involved in important nuclear functions such as coordinating PC synthesis with cell-cycle regulation and regulating proliferation of the nucleoplasmic reticulum, an intranuclear membrane network implicated in calcium signaling and nuclear-cytoplasmic transport (4).|
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Western blot - PCYT1A antibody [EPR3941]; All lanes : Anti-PCYT1A antibody [EPR3941] at 1/1000 dilution.Lane 1 : A549 cell lysate.Lane 2 : K562 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 42 kDa.
Immunocytochemistry/ Immunofluorescence - PCYT1A antibody [EPR3941]; TA307257, at 1/100 dilution, staining PCYT1A in HeLa cells by Immunofluorescence.