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Home Antibody All anti-PCNA antibodies

Anti-PCNA TRUEMAB Antibody Clone OTI2G7, Loading Control

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA800875 Mouse monoclonal anti-PCNA antibody, clone 2G7, Loading, clone OTI2G7 (formerly 2G7) 100ul $248 In Stock
LC400929 PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800875 Carrier-free (BSA/glycerol-free) PCNA mouse monoclonal antibody, clone OTI2G7 (formerly 2G7) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human PCNA (NP_002583) produced in E.coli.
Clone NameClone OTI2G7 IsotypeIgG2b
Species ReactivityHuman, Mouse, Rat, Monkey, Dog Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:2000, IF 1:50
Predicted MW Explanation 28.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameATLD2
Database LinkNP_002583
Entrez Gene 5111 Human
Entrez Gene 18538 Mouse
Entrez Gene 25737 Rat
Entrez Gene 718006 Monkey
Entrez Gene 477166 Dog
FunctionThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (RC201741, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunofluorescent staining of HeLa cells using anti-PCNA mouse monoclonal antibody (TA800875, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).
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