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Anti-PCNA TRUEMAB Antibody Clone 2G7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PCNA (NM_002592)
|Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.|
||WB 1:2000; IF 1:50
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1|
Entrez Gene 5111 Human
|The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]. |
|Stem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (RC201741, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of HeLa cells using anti-PCNA mouse monoclonal antibody (TA800875, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).