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Anti-PARK2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324839
  • Rabbit polyclonal Parkin Antibody (C-term)
400ul 325 3-7 Days
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WB(2)
IHC(2)
IF(1)
FC(1)

OriGene Data

ImmunogenThis Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-417 amino acids from the C-terminal region of human Parkin.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens parkin RBR E3 ubiquitin protein ligase (PARK2), transcript variant 1
Alternative NameAR-JP; LPRS2; PDJ; PRKN
Database LinkNP_004553
Function
Related Pathway

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WB Image
Park2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).
WB Image
The anti-Parkin (C-term) Pab is used in Western blot to detect Parkin in mouse kidney tissue lysate.
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IHC Image
Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Confocal immunofluorescent analysis of Parkin Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
FC Image
Parkin Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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