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Home Antibody All anti-PAK1 antibodies

Anti-PAK1 Antibody EP656Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303623
  • Rabbit monoclonal antibody against PAK1 (EP709Y)
100ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the human PAK1 protein was used as immunogen.
Clone NameEP656Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10,000-50,000; IHC: 1: 250-500; ICC: 1:250-500; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 2
Alternative NamePAKalpha
Database LinkNP_002567
FunctionPAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PAK1 antibody [EP656Y]; Anti-PAK1 (phospho S144) antibody [EP656Y] at 1/50000 dilution + HeLa cell lysate.Predicted band size : 65 kDa.Observed band size : 65 kDa.
WB Image
Western blot - PAK1 (phospho S144) antibody [EP656Y]; All lanes : Anti-PAK1 (phospho S144) antibody [EP656Y].Lane 1 : HeLa Cell lysate.Lane 2 : HeLa Cell lysate treated with lambda phosphatase.Predicted band size : 65 kDa.Observed band size : 65 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - PAK1 (phospho S144) antibody [EP656Y]; Immunohistochemical analysis of paraffin-embedded human colon cancer using TA303623.
IF Image
Immunocytochemistry/ Immunofluorescence - PAK1 (phospho S144) antibody [EP656Y]; Immunofluorescent staining of HeLa cells using TA303623.
FC Image
Flow Cytometry - Anti-PAK1 (phospho S144) antibody [EP656Y]; Overlay histogram showing HeLa cells stained with TA303623 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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