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Anti-PABPN1 Antibody EP3000Y
Also for PABPN1 (NM_004643)
|A synthetic peptide corresponding to residues near the N-terminus of human PABP2 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens poly(A) binding protein, nuclear 1 (PABPN1)|
|OPMD; PAB2; PABII; PABP-2; PABP2|
|This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. An expansion of the trinucleotide (GCG) repeat from normal 6 to 8-13 at the 5' end of the coding region of this gene leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Multiple splice variants have been described but their full-length nature is not known. One splice variant includes introns 1 and 6 but no protein is formed. [provided by RefSeq]. |
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Western blot - PABPN1 antibody [EP3000Y]; Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] at 1/200000 dilution.Lane 3 : Anti-PABPN1 antibody [EP3000Y] at 1/1000000 dilution.Lane 1 : Raw264.7 cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 33 kDa.Observed band size : 49 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y]; TA303741, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y]; TA303741, at 1/100 dilution, staining PABPN1 in HeLa cells, by Immunofluorescent staining.
Flow Cytometry - Anti-PABPN1 antibody [EP3000Y]; Overlay histogram showing MCF-7 cells stained with TA303741 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.