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Anti-Nos1 PHOSPHO Antibody
Also for Nos1 (NM_052799)
|The antiserum was produced against synthesized phosphopeptide derived from human n-NOS around the phosphorylation site of serine 847 (F-N-SP-V-S).|
|Human: Ser852, Mouse: Ser847, Rat: Ser847
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|n-NOS (Phospho-Ser852) antibody detects endogenous levels of n-NOS only when phosphorylated at serine 847.
|Rattus norvegicus nitric oxide synthase 1, neuronal (Nos1)|
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Western blot analysis of extracts from COS7 cells, using n-NOS (Phospho-Ser852) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using n-NOS (Phospho-Ser852) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) antibody.The picture on the right is treated with the synthesized peptide.