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Anti-Ndc80 Antibody EPR5342
Also for Ndc80 (NM_006101)
|A synthetic peptide corresponding to residues near the C-terminus of human Ndc80 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens NDC80 kinetochore complex component (NDC80)|
|HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3|
|Ndc80 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved (1).|
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Western blot - HEC1 antibody [EPR5342]; All lanes : Anti-HEC1 antibody [EPR5342] at 1/10000 dilution.Lane 1 : Jurkat lysate.Lane 2 : HeLa lysate.Lane 3 : NCCIT lysate.Lane 4 : Human spleen lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 74 kDa.