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Anti-NUBPL TrueMAB Antibody clone 5F8
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for NUBPL (NM_025152)
|Human recombinant protein fragment corresponding to amino acids 1-250 of human NUBPL(NP_079428) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB 1:500~2000, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens nucleotide binding protein-like (NUBPL), nuclear gene encoding mitochondrial protein, transcript variant 1|
|C14orf127; huInd1; IND1|
|This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. The respiratory complex I consists of 45 subunits and 8 iron-sulfur (Fe/S) clusters. This protein is an Fe/S protein that plays a critical role in the assembly of respiratory complex I, likely by transferring Fe/S into the Fe/S-containing complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding distinct isoforms have been identified.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUBPL (RC204385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUBPL.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NUBPL monoclonal antibody.
HEK293T cells transfected with either RC204385 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUBPL antibody(TA503729), and then analyzed by flow cytometry.