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Home Antibody All anti-NTRK1 antibodies

Anti-NTRK1 Antibody EP1058Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301109
  • Rabbit Monoclonal Antibody against NTRK1 (Clone EP1058Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419273) , 20ug
100ul 325 In Stock
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WB(2)
IHC(2)
IF(2)
FC(1)
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Also for NTRK1 (NM_002529)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 791 of human Trk A was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated Trk A.
Clone NameEP1058Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; ICC: Use at an assay dependent concentration; WB: 1:2500 - 1:5000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2
Alternative NameMTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA
Database LinkNP_002520
FunctionThe Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).
Related Pathway
MAPK signaling pathway

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WB Image
Western blot - TrkA antibody [EP1058Y]; All lanes : Anti-TrkA antibody [EP1058Y] at 1/5000 dilution.Lane 1 : Rat brain cell lysate un-treated.Lane 2 : Rat brain cell lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 145 kDa .
WB Image
Western blot - Anti-TrkA antibody [EP1058Y]; All lanes : Anti-TrkA antibody [EP1058Y] at 1/2500 dilution.Lane 1 : Human neuronal cell lysate (untreated).Lane 2 : Human neuronal cell lysate (treated with rh-?NGF at 25ng/ml).Lane 3 : Human neuronal cell lysate (treated with rh-?NGF at 50ng/ml).Lane 4 : Human neuronal cell lysate (treated with rh-NT3 at 50ng/ml).Lane 5 : Human neuronal cell lysate (treated with rh-BDNF 50ng/ml).Lane 6 : TrkA protein.Lysates/proteins at 15 µg per lane.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 87 kDa.Observed band size : 145 kDa .Exposure time : 20 minutesThis image is courtesy of an anonymous Abreview
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TrkA antibody [EP1058Y]; Immunohistochemical analysis of TrkA in paraffin embedded human brain tissue using TA301109 at a 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA antibody [EP1058Y]; TA301109 staining TrkA in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). .Tissue was fixed with formaldehyde, permeabilized with 0.1% Saponin/PBS and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/150 in blocking buffer) for 16 hours at 4°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - TrkA antibody [EP1058Y]; Immunofluorescent staining of TrkA in SHSY5Y cells using TA301109 at a 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-TrkA antibody [EP1058Y]; ICC/IF image of TrkA staining on culture of mouse DRG neurons using TA301109 (1:100). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% Goat serumfor 1 hour at 22°C.TA301109 was diluted 1:100 using PBS and incubated with the cells for 30 mins at 22°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to Alexa Fluor 488 (1:1000). Neuron was stained using Beta III tubulin antibody (Alexa Fluor 647)
FC Image
Flow Cytometry - TrkA antibody [EP1058Y]; Overlay histogram showing SH-SY5Y cells stained with TA301109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

 

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