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Anti-NTRK1 Antibody EP1058Y
Also for NTRK1 (NM_002529)
|A synthetic peptide corresponding to residues surrounding tyrosine 791 of human Trk A was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated Trk A.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; ICC: Use at an assay dependent concentration; WB: 1:2500 - 1:5000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2|
|MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA|
|The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3). |
MAPK signaling pathway
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Western blot - TrkA antibody [EP1058Y]; All lanes : Anti-TrkA antibody [EP1058Y] at 1/5000 dilution.Lane 1 : Rat brain cell lysate un-treated.Lane 2 : Rat brain cell lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 145 kDa .
Western blot - Anti-TrkA antibody [EP1058Y]; All lanes : Anti-TrkA antibody [EP1058Y] at 1/2500 dilution.Lane 1 : Human neuronal cell lysate (untreated).Lane 2 : Human neuronal cell lysate (treated with rh-?NGF at 25ng/ml).Lane 3 : Human neuronal cell lysate (treated with rh-?NGF at 50ng/ml).Lane 4 : Human neuronal cell lysate (treated with rh-NT3 at 50ng/ml).Lane 5 : Human neuronal cell lysate (treated with rh-BDNF 50ng/ml).Lane 6 : TrkA protein.Lysates/proteins at 15 µg per lane.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 87 kDa.Observed band size : 145 kDa .Exposure time : 20 minutesThis image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TrkA antibody [EP1058Y]; Immunohistochemical analysis of TrkA in paraffin embedded human brain tissue using ab76291 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA antibody [EP1058Y]; ab76291 staining TrkA in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). .Tissue was fixed with formaldehyde, permeabilized with 0.1% Saponin/PBS and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/150 in blocking buffer) for 16 hours at 4°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - TrkA antibody [EP1058Y]; Immunofluorescent staining of TrkA in SHSY5Y cells using ab76291 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TrkA antibody [EP1058Y]; ICC/IF image of TrkA staining on culture of mouse DRG neurons using ab76291 (1:100). The cells were fixed using formaldehyde and permeabilized using 0.2% Triton X-100. The cells were blocked using 10% Goat serumfor 1 hour at 22°C.ab76291 was diluted 1:100 using PBS and incubated with the cells for 30 mins at 22°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to Alexa Fluor 488 (1:1000). Neuron was stained using Beta III tubulin antibody (Alexa Fluor 647)
Flow Cytometry - TrkA antibody [EP1058Y]; Overlay histogram showing SH-SY5Y cells stained with ab76291 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.