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Home All anti-Nphs1 antibodies
Anti-Nphs1 Phospho Antibody EPR2883Y
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for Nphs1 (NM_004646)
OriGene Data
| A synthetic peptide corresponding to residues surrounding tyrosine 1176 and 1193 of mouse Nephrin was used an immunogen. This antibody detects both Nephrin phosphorylated on tyrosines 1176 and 1193 as well as non-phosphorylated Nephrin. |
| EPR2883Y |
Rabbit IgG |
| Human |
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| WB |
WB: 1: 10,000 - 50,000, |
| Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) |
| CNF; nephrin; NPHN |
| NP_004637 |
| Nephrin (NPHS1) is an immunoglobulin family adhesion protein expressed in visceral glomerular epithelial cells (podocytes), where it is targeted to the intercellular slit diaphragm of mature foot processes (1). It is an essential component of the kidney filtration barrier, and mutations in the nephrin gene are associated with congenital nephrotic syndrome (2). Nephrin binds via phosphorylated tyrosine residues in the intracellular domain to SH2 domain-containing signaling molecules such as Nck (3). |
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* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
A. Western blot analysis on (A) HEK293T cells transfected with wild type CD 16/7 Nephrin (B) HEK293T cells transfected with active tyrosine kinase (vSrc) (C) HEK293T cells transfected with Nephrin mutated at Tyr1176 and vSrc and (D) HEK293T cells transfected with Nephrin mutated at Tyr 1193 and vSrc using Phospho Nephrin (pY1176/1193) RabMAb .
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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