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Home Antibody All anti-NMNAT1 antibodies

Anti-NMNAT1 TRUEMAB Antibody Clone OTI1G4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502220 NMNAT1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) 100ul $379 In Stock
LC402948 NMNAT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502220 Carrier-free (BSA/glycerol-free) NMNAT1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) 100ug $450 3-4 weeks
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WB(2)
IHC(8)
FC(1)
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Also for NMNAT1 (NM_022787)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NMNAT1 (NP_073624) produced in HEK293T cell.
Clone NameClone OTI1G4 IsotypeIgG1
Species ReactivityHuman Concentration0.67 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 31.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nicotinamide nucleotide adenylyltransferase 1 (NMNAT1), transcript variant 1
Alternative NameLCA9; NMNAT; PNAT1
Database LinkNP_073624
Entrez Gene 64802 Human
FunctionThe coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM].
Related Pathway Nicotinate and nicotinamide metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NMNAT1 (RC204825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NMNAT1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NMNAT1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NMNAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502220)
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HEK293T cells transfected with either RC204825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NMNAT1 antibody(TA502220), and then analyzed by flow cytometry.
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