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Home All anti-Nlrp10 antibodies
Also for Nlrp10 (NM_175532)
Mus musculus NLR family, pyrin domain containing 10 (Nlrp10)
6430548I20Rik; Nalp10; Napl10; PAN5; Pynod
Peptide with sequence NDLEENSFKTLKFH-C, from the N Terminus of the protein sequence according to NP_780741.1.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (
Protein A or G Sepharose)
WB: 1-3ug/ml, IF: 0.5-1ug/ml
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Data provided by customer: (1ug/ml) staining of Mouse Embryonic Fibroblasts lysate (25ug protein in RIPA buffer) and in HEK293 overexpressing Mouse Nalp10 (Lane 3), Human NALP10 (Lane 2) and empty vector (Lane 1). . Primary incubation was 1 hour. Detected by chemiluminescence.
Data provided by customer: (5ug/ml) staining of Mouse Embryonic Fibroblasts (red), and of HeLa overexpressing Mouse Nalp10. Nuclear staining by DAPI (blue).